Associate Professor of Oncology
Department of Cancer Genetics

Director, SKY/FISH Facility

Roswell Park Cancer Institute
Elm and Carlton Streets
Buffalo, NY 14263
Telephone: 716-845-3213
Fax: 716-845-1698
E-mail: Seiichi.Matsui@RoswellPark.org

Background

Dr. Matsui joined Roswell Park Cancer Institute in 1976 and has been serving as Director of the SKY Resource since 2000. His expertise encompasses genetics, cytogenetics, biochemistry, enzymology, pharmacology, molecular and cell biology. Dr. Matsui invented N-banding techniques which allow in situ detection of rDNA-bound non-histone chromosome proteins. He also discovered and characterized isopeptidase (also dubbed ‘ubiquitin-deconjugation enzyme’) which has been implicated in Parkinson’s disease, tumor suppressors, androgen signaling, synapsis function, etc. ‘Isopeptidase’, the term christened by Dr. Matsui, is widely accepted by the scientific community with citations in more than 66,000 articles (Google search as of Sept. 2007). He was the Assistant Editor of Cancer Genetics and Cytogenetics for 10 years.  He served on an NIH Study Section for evaluation of the Cancer Center Program Grants. He was also an external grant reviewer for the National Science Foundation and the Medical Research Council in Canada.

Description of Research

• Genomic instability of cancer. Its magnitude ranges from chromosomal level to nucleotide level. Our interest is focused on identification of chromosomal changes which alter the expression of genes which in turn causes pathological conditions associated with malignancy
  
• Collaborating with DNA Microarray and Gene Expression Resources as well as by inter-institutional collaborations, we are attempting to define underlying mechanisms of genomic instability. A discovery that DNA methyltransferases are highly involved in chromosomal instability highlights one of our recent accomplishments.

Key Publications

• Rossi M, LaDuca J, Cowell J, Srivastava BIS, Matsui S.  Genomic analysis of CD8+ NK/T cell line with array-based CGH(aCGH), SKY/FISH and molecular mapping.  Leukemia Res, In Press.
• Desler C, Munch-Peterson B, Stevnsner T, Matsui S, Kulawiec M, Singh KK, Rasmussen LJ.  Mitochondria as determinant of nucleotide pools and chromosomal instability. Mutat Res, In Press.
• Head K, Gong S, Joseph S, Wang C, Burkhardt T, Rossi MR, LaDuca J, Matsui S-I, Vaughan M, Hicks DG, Heintz N, Cowell JK.  Defining the expression pattern of the LGI1 gene in BAC transgenic mice.  Mamm Genome 2007; 18(5):328-337.
• Nestor AL, Hollopeter SL, Matsui S, Allison DC.  Genetic complementation may explain the structurally abnormal chromosmes and LOHs of cancer aneuploidy.  Cytogenet Genome Res 2007; 116:235-247.
• Stoler DL, Nowak NJ, Matsui S-I, Wiseman SM, Chen N, Dutt S, Bartos JD, Loree TR, Rigual NR, Hicks WL, Sait SN, Anderson GR.  Comparative genome instabilities of thyroid and colon cancers.  Arch Otolaryngol Head Neck Surg 2007; 133:457-463.
• Li Z, Zu T, Morishima M, Pao A, Laduca J, Controy J, Nowak N, Matsui SI, Shirasishi I, Yu YE.  Dupllication of the entire 2Mb human chromosome 21 syntenic region on mouse chromosome 16 causes cardiovascular and gastrointestinal abnormalities.  Hum Mol Genet 2007;16(11):1359-1366.
• Mason DX, Zhang J, Keppler D Jackson TJ, Seger Y, Matsui S, Cowell JK, Hannon G J, Lowe S W, Lin AW. Defined genetic events associated with the spontaneous in vitro transformation of E1A/Ras-expressing human IMR90 fibroblasts. Carcinogenesis 2006;  2:350-359.
• Loewen GM, Tracy E, Blanchard F, Tan D, Yu J, Raza S, Matsui S, Baumann, H. Transformation of human bronchial epithelial cells alters sponsiveness to inflammatory cytokines. BMC Cancer 2005; 5:145.
• Cowell JK, LaDuca J, Rossi MR, Burkhardt T, Nowak NJ, Matsui S. Molecular characterization of the t(3;9) translocation associated with immortalization in the MCF10A cell line. Cancer Genet Cytogenet 2005; 163:23:29.
• Rossi MR, LaDuca J, Matsui S, Nowak NJ, Hawthorn L, Cowell JK. Novem amplicons on the short arm of chromosome 7 identified using high resultion array contain overexpressed genes in addition to EGFR in glioblastoma multiform. Genes Chromosomes Cancer 2005; 44:392-404.
• Maitra A, Arking DE, Shivapurkar N, Ikeda M, Stasny V, Kassauei K, Suit G, Gutler DJ, Liu Y, Brimble SN, Noaksson K, Hyllner J, Schultz TC, Zeng X, Freed WJ, Crook J, Abraham S, Colman A, Sartipy P, Matsui S, Carpenter M, Gazdar AF, Rao M, Chakravarti A. Genomic alterations in cultured human embronic stem cells. Nat Genet 2005; 10:1099-1103.
• Rossi MR, Gaile D, LaDuca J, Matsui S, Conroy J, McQuaid D, Chervinsky D, Eddy R, Chen H-S, Barnett GH, Nowak NJ, Cowell JK. Identification of consistent novel submegabase deletions in low-grade oligodendrogliomas using array-based comparative genomic hybridization. Genes Chromosomes Cancer 2005; 44:85-96.
• Singh KK, Kulaweic M, Still I, Desouki MM, Geradts J, Matsui S. Inter-genomic cross talk between mitochondria and the nucleus plays an important role in tumorigenesis. Gene 2005; 354:140-146.
• Karpf AR and Matsui S. Genetic disruption of cytosine DNA methaltransferase enzymes induces chromosomal instability in human cancer cells. Cancer Res 2005; 65:8635-8639.
• Tu LC, Matsui S, Beerman TA. The monofunctional DNA alkylator hedamycin induces persistent DNA breaks that activate H2AX in a PIKK independent manner and does not require DNA replication fork movement. Mol Cancer Therap 2005; 4(8):1175-1185.
• McHugh MM, Gawron LS, Matsui S, Beerman TA. The antitumor?enediyne C-1027 alters cell cycle progression and induces chromosomal aberrations and telomere dysfunction. Cancer Res 2005; 65:5344-5351.
• Bartos JD, Stoler D L, Matsui S, Swede H, Willmott L J, Sait SN, Petrelli NJ, Anderson GR. Genomic heterogeneity and instability in colorectal cancer: Spectral Karyotyping, glutathione transferase-M1 and Ras. Mutat Res 2004; 568:283-292.
• Matsui S, LaDuca J, Rossi MR, Nowak NJ, Cowell JK. Molecular characterization of a Consistent 4.5 Megabase Deletion in 4q28 in Prostate Cancer Cells. Cancer Genetics & Cytogenetics. Cancer Genet Cytogenet 2004; 159:18-26.
• Cowell JK, Matsui SI, Wang J, LaDuca J, Conroy J, McQuaid D, Nowak NJ. Application of comparative genome hybridization using BAC arrays (CGHa) and spectral karyotyping (SKY) to the analysis of glioblastoma multiforme. Cancer Genet Cytogenet 2004; 151:36-51.
• Ionov Y, Matsui S, Cowell JK. A role for the P300/CBP genes in promoting cancer Progression in colon cancer cell lines with microsatellite instablility. Proc Natl Acad Sci USA 2004; 101:1273-1278.
• Iyer RV, Sait SN, Matsui SI, Block AW, Barcos M, Slack NL, Wetzler M, Baer MR. Massive hyperdiploidy and tetraploidy in acute myelocytic leukemia and myelodysplastic syndrome. Cancer Genet Cytogenet 2004; 148:29-34.
• Matsui SI, Faitar SL, Rossi MR, Cowell JK. Application of spectral karotyping (SKY) to the analysis of the human chromosome complement of interspecies somatic cell hybrids. Cancer Genet Cytogenet 2003; 142:30-35.
• Matsui S, Sait S, Jones CA, Nowak NJ, Gross KW. Rapid localization of transgenes in mouse chromosomes using a combined spectral karyotyping/FISH technique. Mamm Genome 2002; 13:680-685.
• Sait SN, Qadir MU, Conroy J, Matsui S, Nowak NJ, Baer MR. Double minute chromosomes in acute myeloid leukemia and myelodyspastic syndrome: Identification of new amplification regions by fluorescence in situ hybridization and spectral karyotyping. Genes Chromosomes Cancer 2002; 34:42-47.