Department of Immunology
Director of Graduate Studies and Admissions
Molecular and Cellular Biophysics and Biochemistry Graduate Program
Roswell Park Cancer Institute
Elm and Carlton Streets
Buffalo New York USA 14263
Tel: 716-845-8911
Fax: 716-845-8899
E-mail: arindam.sen@roswellpark.org

Education
BSc, Physics, Delhi University, Delhi, India
MSc, Physics, Delhi University, Delhi, India
PhD, Life Sciences, J. Nehru University, N. Delhi, India
Postdoctoral, Biophysics, Chelsea College, University of London, London, UK

Research Interest

My general research interests include structure-function relationship of biological membranes, model membrane systems and biomolecules using biophysical techniques including microscopy, spectroscopy, x-ray and electron diffraction. My specialization is in the area of electron, optical and atomic force microscopy and, uv/vis and infrared spectroscopic techniques. Specific interests include developing strategies for parenteral drug delivery including transdermal, lipidic nanoparticles and macromolecular assemblies as drug delivery systems, and the development of biosensors using planar immobilized assemblies of biomolecules. Further, we are investigating the physical barriers posed by the tumor microenvironment on effective chemo and radiation therapy, and possible approaches for overcoming those barriers.

Current Research

Transdermal Drug Delivery and Analyte Monitoring
The broad aim of this program is to develop technologies that can automate transdermal drug delivery and analyte monitoring with minimal discomfort and inconvenience. Recent transdermal electroporation work in this laboratory has resulted in a novel method using electric pulses and lipid formulations to achieve a transient increase in skin permeability, enabling sampling of systemic glucose and delivery of insulin. We have received an US patent for transdermal monitoring of analytes and have a patent pending for transdermal delivery.

Lipidic Nanoparticles for Drug Delivery
The aim of this program is to design and test novel lipidic nanoparticles for drug delivery to tumors with the aim of translating this program to the clinic. Amongst the lipidic nanoparticles that we have tested include those that are temperature sensitive for which we have received an US patent. Research is aimed at tumor targeting of nanoparticles by using hyperthermia and other adjuvant therapies to enhance the therapeutic efficacy in difficult to treat solid tumors including metastatic cancers.

Tumor Microenvironment
The aim of this program is to examine changes in the tumor vasculature and microenvironment in response to hyperthermia or other therapies that can influence tumor drug delivery. Our aim is to establish role of different adjuvant treatments like hyperthermia in increasing tumor uptake of drugs possibly by remodeling tumor vasculature and/or by inducing changes in tumor hydrostatic pressure.

Development of biosensors
This is a new program in which our aim is to develop novel, label-free spectroscopic and molecular imaging methods for rapid detection of biological agents for diagnosis of different diseases and infectious agents.

Patents

1. Method for increasing the efficiency of transfection. Patent No. US 6,187,588 B1 Inventors: S.W. Hui, S.P. Murphy, L. H. Li and A. Sen
2. Method for transdermal sampling of analytes. Patent No. US 6,383,138 B1 Inventors: A. Sen, S.W. Hui and Y.L. Zhao
3. Temperature controlled content release from liposomes. Patent No. US 6,964,778 B1. Inventors: S.W. Hui and A. Sen.
4. Temperature-sensitive control of liposomes-cell adhesion. Patent No. US 6,991,805B1. Inventors: S.W. Hui and A. Sen.
5. Method for transdermal or intradermal delivery of molecules. Patent pending. Serial No. 60/184,918. Inventors: A. Sen, S.W. Hui, Y.L. Zhao and L. Zhang.

Selected Recent Publications

• Sen, A., Zhao, Y.L. Zhang, L and Hui, S.W. (2002) Enhanced transdermal transport by electroporation using anionic lipids. J. Controlled Rel. 82(2-3): 399-406.
• Sen, A., Zhao, Y.L. and Hui, S.W. (2002) Saturated anionic phospholipids enhance transdermal transport by electroporation. Biophys. J. 83: 2064-2073.
• Sen, A., Daly, M.E. and Hui, S.W. (2002) Transdermal Insulin Delivery Using Lipid Enhanced Electroporation. Biochim. Biophys. Acta 1564: 5-8.
• Wells, J., Sen, A. and Hui, S.W. (2003) Localized delivery to CT-26 tumors in mice using thermosensitive liposomes. Intl. J. Pharmaceutics 261: 105-114.
• Murthy, S. N., Sen, A., Zhao, Y., and Hui, S.W. (2003) pH influences the post-pulse permeability state of skin after electroporation. J. Controlled Rel. 93: 49-57.
• Murthy, S.N., Sen, A., Zhao, Y and Hui, S.W. (2004) Temperature influences the post-pulse permeability state of skin after electroporation. J. Pharmaceutical Sci. 93: 908-915.
• Murthy, S.N., Sen, A., Zhao, Y. and Hui, S.W. (2004) Surfactant-enhanced transdermal delivery by electroporation: J. Controlled Rel. 98: 307-315.
• Murthy, S.N., Zhao, Y., Hui, S.W. and Sen, A. (2005) Electroporation and transcutaneous extraction (ETE) for pharmacokinetics studies of drugs. J. Controlled Rel. 105: 132-141.
• Murthy, S.N., Zhao, Y., Hui, S.W. and Sen, A. (2006) Lipid and electroosmosis enhanced transdermal delivery of insulin by electroporation. J. Pharmaceutical Sci. 95, 2041-4050.
• Murthy, S.N., Zhao, Y., Hui, S.W. and Sen, A. (2006) Synergistic effect of lipid treatment and electroosmosis for transdermal delivery of insulin. Intl. J. Pharm. 326: 1-6.
• Burgess, S.E., Zhao, Y. Sen, A. and Hui, S.W. (2007) Resealing of electroporation of porcine epidermis using phospholipids and poloxamers. Intl. J. Pharm. 336: 269-275.
• Xu, Y., Choi, J., Sen, A., Hylander, B. L., Evans, S. S. Kraybill, W. G. and Repasky, E. A. (2007) Fever-range whole body hyperthermia enhances the delivery and therapeutic efficacy of liposomally encapsulated doxorubicin by specific functional alterations in tumor vasculature. Intl. J. Hyperthermia 23: 513-527.
• Sckolnick, M., Hui, S.W. and Sen A. (2008) Influence of DMPS on the water retention capacity of porcine skin. Intl. J. Pharm. 350: 138-144.