Research Associate, Department of Cancer Biology
Roswell Park Cancer Institute 
Elm and Carlton Streets
Buffalo, New York USA 14263
Telephone: (716) 845-8302
Fax: (716) 845-1349
E-mail: thamarapu.srikrishnan@roswellpark.org

Education
Vivekanada College, Madras, India, BSc, 1962, Physics
Presidency College, Madras, India, MSc, 1964, Physics
University of Madras, Madras, India, PhD, 1969, Biophysics

General Research Interest
My research activities currently are focused on the following four projects:

1. Characterization of the binding avidity of Alzheimers protein (Ab4) and its fragments on TIC;
2. Thiophilic interaction chromatography of Lactoferrin and human transferrins;
3. Structure and conformational investigations of tandem lesions of DNA;
4. Structural studies of HSA complexes with Porphyrin photosensitizers;
5. Structure and conformation of Interleukin-15 (IL-15), and
6. Structure-activity studies of transplant binding protein, Smp50.

In addition, our accomplishments are in the areas of the conformation of modified nucleic acid components, nucleotides and nucleosides; nucleic acid-drug interactions; role of water in biological systems; structure-activity studies of immunosuppressive agents; structure and conformation of crystalline oligosaccharides; studies on bio-active peptides; studies on carcinogens and compounds of interest in cancer chemotherapy and the use of physico-chemical techniques in understanding protein structures.

Key Publications

• Xia, J., Srikrishnan, T., Alderfer, J.L., Jain, R.K., Piskorz, C.F. and Matta, K.L.: Synthesis of sulfated Oligosaccharides with b-D-Gal-1,3 [b-D-Gal 1,4 (a-L-Fuc 1,3)] b-D-GlcNAc 1,6 a-D-Gal N AcOme sequence. Carb. Res. 329 (3): 561-577, (2000).
• Dasari, K. and Srikrishnan, T.: Crystal structure and conformation of 2,7,-bis(piperinopropoxy)-9H-fluoren-9-one:An antiviral agent and an interferon inducer. J. Chem. Crystallography 30: 269-273, 2000.
• Todorova, D., Srikrishnan, T. and Vijayalakshmi, M.A.: Copper binding to prion octarepeat peptides, a combined IMAC and immunochemical approach. Paper presented at the 20th International symposium on the separation and analysis of Proteins, peptides & Polynucloetides held at Ljubljana, Slovenia, 2000.
• Mettath, S., Li, G., Srikrishnan, T., Mehta, R., Grossman, D., Dougherty, T. and Pandey, R.K.: Effect of substituents in directing the formation of benzochlorins and isobacteriochlorins in Porphyrin and Chlorin systems. Organic Letters. 1: 1961-1964, 1999.
• Rooth, W. and Srikrishnan, T.: Crystal structure and conformation of Frentizole, [1-(6-methoxy-2-benzothiazolyl)-3-phenylurea, an antiviral agent and an immunosuppressive drug. J. Chemical Crystallography 29:1120-1125, 1999.
• Sapico, LF., Gesme, J., Iijima, H., Paxton, R. and Srikrishnan, T.: Solution studies of Interleukin-15 reveal a tertiary fold different than IL-2. J. Biosciences. 24: 44, 1999.
• Srikrishnan, T.: Tandem lesions in DNA oligomers and duplxes: Detailed analysis of their structure, conformation and energetic studies. J. Biosciences 24: 91, 1999.
• Penoyar, J., Srikrishnan, T. and LoVerde, P.T.: Secondary structural studies of a Schistosoma mansoni protein (Smp50) using circular dichroism (CD) and Fourier Transform Infra-red spectroscopic techniques. J. Biosciences 24: 71, 1999.
• Srikrishnan, T.: Crystal structure and molecular conformation of 4-thiouracil-2'-trifluorothioacetamide-3',5'-diacetyl-ß-D-riboside. Nucleosides & Nucleotides, 17, 2211-2220 (1998).
• Srikrishnan, T.: Structure, conformation and energetic studies of tandem lesions of DNA induced by reactive oxygen species. In: Proceedings of the XVIIth International Cancer Congress held at Rio de Janeiro, Brazil, August 22-29, 1998 published by Monduzzi Editore, Bologna, Italy, pp. 189-195 (1998).
• Kozyrev, A.N., Alderfer, J.L., Srikrishnan, T., Dougherty, T.J. and Pandey, R.K.: Synthesis of novel laterally-bridged pyropheophorbide-a dimers. J. Chem. Soc., Perkin Trans. 1: 837-838 (1998).
• Belletti, J., Ramasubbu, N., Dougherty, T.J., Pandey, R.K. and Srikrishnan, T.: Investigation of the relative binding specificities of three novel porphyrin photosensitizer drugs to human serum albumin (HSA) protein using circular dichroism (CD) and UV-VIS spectroscopic techniques. Biophys. J.74: A104 (1998).
• Srikrishnan, T., Sen, A. and Loverde, P.: Investigation of the structure of schistosoma mansoni protein (Smp50) by circular dichroism (CD) and Fourier transform infra-red spectroscopy (FTIR) spectroscopic techniques. Biophys. J. 74: A105 (1998).
• Parthasarathy, R. and Srikrishnan, T.: Crystal structure of a non-characteristic ribose conformation of a cyclic nucleotide: Structure of 3', 5' cyclic CMP. Nucleosides & Nucleotides 16: 1967-1981 (1997).
• Arakali, A.V., McCloskey, R., Parthasarathy, R., Alderfer, J.A., Chheda, G.B. and Srikrishnan, T.: Study of creatinine and its 5-alkoxy analogs: Structure and conformational studies in the solid and solution states by X-ray crystallography, NMR, UV and Mass Spectroscopy. Nucleosides & Nucleotides 16: 2193-2218 (1997).
• Srikrishnan, T., Iijima, H., Balakrishnan, R., Penoyar, J. and Loverde, P.: Analysis of the secondary structure of schistosoma mansoni protein (Smp 50) using circular dichroism spectroscopy. Biophys. J. 72: A391 (1997).
• Sapico, L., Gesme, J., Iijima, H., Balakrishnan, R., Paxton, R. and Srikrishnan, T.: Investigation of the secondary structures of simian and human interleukin 15 (IL-15) using circular dichroism spectroscopy. Biophys. J. 72: A31 (1997).
• Srikrishnan, T., Parthasarathy, R., Pfleiderer, W., De Nimai, C. and Chheda, G.B.: Cis-trans isomerization of the N-formyl amino group in a novel modified uracil derivative: 6-amino-3-methyl-5-(N-formylamino)uracil. Nucleosides & Nucleotides 16: 239-255 (1997).
• Hoffman, S. and Srikrishnan, T.: Structure and conformation of sequentially related peptides: Crystal structure of phenylalanylglycylglycine (FGG). Biophys. J. 70: A104 (1996).
• Soni, S.D., Srikrishnan, T. and Alderfer, J.L.: Structure and conformation of 5-fluorocytidine, an anti-tumor agent as determined from NMR and X-ray diffraction data. Nucleosides & Nucleotides 15: 1945-1957 (1996).
• Srikrishnan, T., Kanthi, B. Dasari, K., Zaleski, M. and Albini, B.: Structural and functional studies of immunomodulators: Preliminary proliferation studies on FK-506, tilorone and LS2616. In: Proceedings of the Interntional Cancer Congress held at New Delhi, India, Oct. 30-Nov. 5, 1994, published by Monduzzi Editore, Bologna, Italy, Vol. 4, pp. 2817-2121.
• Puius, Y., Steivater, T. and Srikrishnan, T.: Structure and conformational studies of crystalline antibiotics: Molecular structure and absolute configuration of Kanamycin A. Acta Cryst. A49: 130 (1993).
• Dasari, K. and Srikrishnan, T.: Studies on the structure and conformation of Trimethoprim: X-ray investigation of the drug with three conformers in the crystalline state. Acta Cryst. A49: 146 (1993).
• Srikrishnan, T., Dasari, K. and Albini, B.: Crystal structure and immunosuppressive activity of Tilorone, an interferon inducer: Structure of 2,7-bis(piperinopropoxy)-9H-fluoren-9-one. Acta Cryst. A49: 130 (1993).
• Kanthi, B., Dasari, K. and Srikrishnan, T.: Crystal structure and conformation of 2,7-bis[piperinopropoxy]-9H-fluoren-9-one,an analog of tilorone. Biophys. J. 59:115a (1991).
• Kanthi, B., Dasari, K. and Srikrishnan, T.: Structural studies of immunomodulators:crystal structure of Linomide (LS-2616), a new immunomodulator. Amer. Cryst. Mtg. Program and Abstracts. 2, 19, 90 (1991).
• Srikrishnan, T. and Parthasarathy, R.: Structure and conformation of N-formylpeptides : Crystal and molecular structure of N-formyl-L-alanyl-L-aspartic acid. Int. J. Pep. & Prot. Res. 38: 335 (1991).
• Srikrishnan, T.: Structural studies of immunomodulators. Part II: Crystal structure and conformation of Azimexon (BM12531), an immunostimulant and an anticancer drug. Anticancer Drug Design 5: 213 (1990).
• Wood, C.L., Rooth, W.L. and Srikrishnan, T.: Structural studies of immunomodulators and other related drug molecules. Crystal and molecular structures of Frentizole and Azimexon. Acta Cryst. A46, C160 (1990).
• Srikrishnan, T. and Parthasarathy, R.: Crystal structure of N-methylnicotinamide. Acta Cryst. C46, 1723 (1990).
• Srikrishnan, T.: Crystal structure and conformation of Perphenazine, conformational variabilities of the phenothiazine ring. Biophys. J. 55: 363a (1989).
• Srikrishnan, T., Chowdhary, M. and Matta, K.L.: Crystaland molecular structure of O-a-D-mannopyranosyl-(1,2)-O-methylmannopyranose. Carb. Res. 186: 167 (1988).
• Srikrishnan, T. and An, S.H. Crystal structure of 1,3,4,6,-tetra-O-acetyl-2-deoxy-2-fluoro-ß-D-galactoside. J. Carb. Chem. 7: 571 (1988).
• Parthasarathy, R., Fridey, S.M. and Srikrishnan, T.: Conformation and hydrogen bonding of N-formylmethionyl peptides II: Crystal and molecular structure of N-formyl-L-methionyl-l-Phenylalanine. Int. J. Pep. & Prot. Res. 33: 308 (1988).
• Srikrishnan, T. Ravichandran, V. and Chacko K.K.: Conformation of methylated amino acids: Crystal and molecular structure of 3,4-dimethoxy-a-methyl-DL-Phenylalanine sesquihydrate. Acta Cryst. C44: 847-850 (1988).
• Srikrishnan, T., Parthasarathy, R., Alderfer, J.A., Dutta, S.P. and Chheda, G.B.: Structure and conformation of 1-ß-D-ribofuranosyl pyridin-4-one-3-carboxamide, a novel nucleoside from human urine with a rare ribose pucker. Nucleosides and Nucleotides 7: 45-60 (1988).